Plasmid_Backbone

Part:BBa_K4212067:Design

Designed by: Youzi Bi   Group: iGEM22_Imperial_College_London   (2022-10-03)


STK-EXP-1-sfGFP


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Plasmid lacks a prefix.
    Plasmid lacks a suffix.
  • 12
    INCOMPATIBLE WITH RFC[12]
    Plasmid lacks a prefix.
    Plasmid lacks a suffix.
  • 21
    INCOMPATIBLE WITH RFC[21]
    Plasmid lacks a prefix.
    Plasmid lacks a suffix.
  • 23
    INCOMPATIBLE WITH RFC[23]
    Plasmid lacks a prefix.
    Plasmid lacks a suffix.
  • 25
    INCOMPATIBLE WITH RFC[25]
    Plasmid lacks a prefix.
    Plasmid lacks a suffix.
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Plasmid lacks a prefix.
    Plasmid lacks a suffix.
    Illegal BsaI site found at 3473
    Illegal BsaI.rc site found at 2455
    Illegal SapI site found at 813
    Illegal SapI.rc site found at 2639


Design Notes

The target gene was inserted in the vector, which could allow rapid, nontoxic selection of successfully transduced cells.


Source

Synthetic construct.

References